Team:TU Darmstadt/Materials/tphC

From 2012.igem.org

Revision as of 20:49, 26 September 2012 by Vali (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808001 tphC]

Primers for the construction of a BioBrick containing the terephtalate periplasmatic binding protein of the tripartite tricarboxylate transporter family.

tphC BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgcgcaacgcatccattcgcag
rev: gtttcttcctgcagcggccgctactagtattagagcttgacgttggcggcc

tphC Pst1

Primers for removing a Pst1 site.

     5'                             3'
fwd: TACATCCCACCTGACGGCAGCCATGTTCATG
rev: CATGAACATGGCTGCCGTCAGGTGGGATGTA