Team:TU Darmstadt/Materials/tphC

From 2012.igem.org

tphC

Primers for the construction of a BioBrick containing the terephtalate periplasmatic binding protein of the tripartite tricarboxylate transporter family.

tphC BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgcgcaacgcatccattcgcag
rev: gtttcttcctgcagcggccgctactagtattagagcttgacgttggcggcc

tphC Pst1

Primers for removing a Pst1 site.

     5'                             3'
fwd: TACATCCCACCTGACGGCAGCCATGTTCATG
rev: CATGAACATGGCTGCCGTCAGGTGGGATGTA