Team:TU Darmstadt/Materials/Kanamycin

Kanamycin

About

Kanamycin is an aminoglycoside antibiotic that interacts with the 30S subunit of prokayotic ribosomes inducing mistranslations thus inhibiting translocation during protein synthesis.^[1]

Stock

1. Mix:
   • 3 g kanamycin (75 mg/mL)
   • ad. 40 mL dist. water
2. sterile filtration
3. aliquot in 1 mL stocks and store at -20°C

Stability

• Kanamycin is one of the few autoclavable antibiotics
• Culture plates can be stored for about 2-3 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm])

References

[1] Pestka, S.: "The Use of Inhibitors in Studies on Protein Synthesis", Methods in Enzymology 30, pp.261-282, 1975