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Team:Freiburg/Project
From 2012.igem.org
Project
Overall project
Since June 2012, we are working in the field of so called TAL effectors in our lab. TAL effectors are molecular components that are fused to DNA binding proteins in order to restrict their acticvity to specific loci in a genome. In particular, we develop a strategy to cheaply create novel TAL effectors in an extremely short period of time. We believe that we have laid a solid foundation for super-easy site specific genome modifications by future iGEM teams.
We hope you will learn something about our project and the power of TAL effectors.
0. Introduction
You don't know what TAL effectors actually are? We reviewed the recent literature for you, to give you a quick overview of this exciting new field of research.
1. Golden Gate Standard
Assembling multiple gene constructs in frame without leaving scars is not possible with existing iGEM standards. We therefore introduce the new Golden-Gate Standard that is fully compatible with RFC 10.
2. The TAL Vector
Targeting a sequence and not doing something to it, is like throwing mechanics at your car. Your car will not get any better only the mechanics will get mad. Because we know this, we bring the tools you need to actually work with DNA.To make it even more easy these tools are deliverd already inside the final TAL backbone, just add the sequence and you're ready.
3. GATE Assembly Kit
We have invented a super-fast, super-easy and super-cheap Method for custom TAL effector construction. Learn about the theory behind the TAL effector toolkit, how we created it and why we choose this design.
4. Using the Toolkit
Our overall goal is to empower future iGEM teams to use the most exciting new technology synthetic biology has to offer. We therefore not only invented the GATE assembly platform but wrote a step by step manual for super-easy custom TALE construction
4. The future TAL projects
Until now, almost three years after deciphering the TALE code, only two types of TAL Effectors have been developed: TALENs and TAL-TFs. We herein propose additional classes of TAL effectors.
5. Experiments and Results
We not only rigorously tested if our in vitro TALE gene assembly method works but also if our TALE constructs actually work in a human cell line. Check out test design and results.
