Team:SDU-Denmark/labwork/Protocols/gel
From 2012.igem.org
mRNA Isolation | PCR | Miniprep | Check Digest |
3A-Assembly | Colony-PCR | Transformation | Gel-electrophoresis |
Reverse Transcriptase | Mutagenisis | PCR-,gel clean-up |
Gel Electrophoresis
Construct a gel - Medium size
1. Weigh 0.8g agarose
2. Mix with 100ml TBEx1 solution
3. Heat in a microwave until all the agarose is dissolved and the solution is clear.
4. Cool it down a bit before adding Ethidium Bromide (warning: toxic)
5. Pour the solution into a gel casting mold and use a comb of desired size.
6. Run at 100V for 40 minutes.