Team:SDU-Denmark/labwork/Protocols/gel

From 2012.igem.org

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenesis PCR-,gel clean-up

Gel Electrophoresis

Construct a gel - Medium size


1) Weigh 0.8g agarose

2) Mix with 100ml TBEx1 solution

3) Heat in a microwave until all the agarose is dissolved and the solution is clear.

4) Cool it down a bit before adding Ethidium Bromide (warning: toxic)

5) Pour the solution into a gel casting mold and use a comb of desired size.

6) Run at 100V for 40 minutes.