Check Digest

Digest mix, using Fastdigest enzymes

1) Cast an agarose gel for purification of the cut product

2) Mix the digestion mixture in an eppendorf tube

3) Leave for 5 min. at 37°C (no shaking!)

4) Immidiately load the restriction mixture in the gel

5) Run the gel, cut out and purify bands of correct size.


Digestion Mix

4 µL H2O 
0,5 µL enzyme A
0,5 µL enzyme B
2 µL Fast Digest green buffer
3 µL dna product