The iGEM Team of University of Southern Denmark 2012 igem.sdu.2012@gmail.com

mRNA Isolation	PCR	Miniprep	Check Digest
3A-Assembly	Colony-PCR	Transformation	Gel-electrophoresis
Reverse Transcriptase	Mutagenisis	PCR-,gel clean-up	Content

Gel Electrophoresis

Construct a gel - Medium size

1. Weigh 0,8g agarose

2. Mix with 100ml TBEx1 solution

3. Heat in a microwave until all the agarose is dissolved and the solution is clear.

4. Cool it down a bit before adding Ethidium Bromide (warning: toxic)

5. Pour the solution into a gel casting mold and use a comb of desired size.

6. Run at 100V for 40 minutes.