Team:HKUST-Hong Kong/Achievement
From 2012.igem.org
Achievement
Our iGEM journey has spanned the length of six months; from the day our project was first proposed in mid-March to the day we ended wet lab work in mid-September.
We are proud to say that within our three months of wet lab work we have achieved the following:
- Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of Bacillus subtilis (BBA_K733007).
- Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM respectively (BBA_K733016, BBA_K733017).
- Successfully constructed a cell growth inhibition device for regulating BMP2 production (BBA_K733012).
- Successfully characterized the low efficiency promoter pTms which was used to drive the expression of antitoxin YdcD (BBA_K733009, BBA_K733018).
- Successfully characterized a xylose-inducible promoter that was used to control the expression of BMP2 and toxin YdcE.
- Successfully characterized the cell growth inhibition device.