Team:ETH Zurich/Decoder/Design

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Navigate through Overview, Design and Results

Dual input promoters

In our case we want the decoder (construct 1 construct 2) to have NOT logics. Therefore we designed 3 different dual input promoters, all of them derived from the λ-phage promoters PR and PL:

1. [http://partsregistry.org/Part:BBa_K909012 Modified PR promoter with operator sites OR1,OR2 and LacO1]

2. [http://partsregistry.org/Part:BBa_K909013 Modified PR promoter with operator sites OR1,OR2 and TetO1]

3. [http://partsregistry.org/Part:BBa_K909011 Modified PL promoter with operator sites TetO1 and LacO1]


Output: p-ABA

We constructed one [http://partsregistry.org/Part:BBa_K909015 pab operon] consisting of the pabA and pabB/C gene from Lactococcus lactis to overexpress p-ABA. Therefore we used the biobrick parts [http://partsregistry.org/Part:BBa_K137055 BBa_K137055] and [http://partsregistry.org/Part:BBa_S04039 BBa_S04039] as a template to construct the wished composites.

Biobrick [http://partsregistry.org/Part:BBa_K909014 K909014] will be used to show that p-ABA is produced, while the [http://partsregistry.org/Part:BBa_K909015 operon] can be cloned downstream to any promoter.

In a second step the pab operon will be cloned downstream of the [http://partsregistry.org/Part:BBa_K909011 PL promoter with operator sites TetO1 and LacO1]. In case blue and red light is present, p-ABA will be produced as a blue and red light response.

Eth2012pabagoal.jpg




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