Question:

Should we separate plasmids from Biobricks? Should each section apply to each construct?

Contents 1 Construction of plasmids 1.1 Assembling DNA parts 1.2 Confirming 1.3 Corroborating sequences 1.4 Conclusions 2 Transforming Synechocystis PCC 6803 2.1 Transformation 2.2 Reestreaking 2.3 Confirming DNA 2.4 Conclusions 3 Revealing phenotypes 3.1 Microscopy 3.2 Luminometer readings 3.3 Conclusions

Construction of plasmids

Assembling DNA parts

Confirming

Corroborating sequences

Conclusions

Transforming Synechocystis PCC 6803

Transformation

Reestreaking

Confirming DNA

Conclusions

Revealing phenotypes

Microscopy

Luminometer readings

Conclusions