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Team:Osaka/Notebook
From 2012.igem.org
Weekly Summaries
Week 1
Went through basic molecular biology lab skills and techniques with the new members. Extracted most of the existing parts required for our project from the iGEM 2012 Spring distribution plates, transformed and amplified the DNA for future use.DNA extraction & purification (miniprep) of transformed parts, & gel runs to confirm lengths. Ligations to put together some common combinations (eg promoter + RBS).
We transfered redioregistance parts from one vector(pSB1C3) to another vector(pSB1A3) and transformed plasmid DNA into Rosetta E.coli.
Week 2
We run the SDS-PAGE gel to check the expression of the gene products at the protein level. The result of DNA sequencing demonstrated that there is a mutation in PprA(Silent mutation).
Week 3
Extraction & amplification of more basic parts from the distribution plates, plus ligation of a few more parts. Not much progress due to lab being closed for Obon holidays.
Week 4
UV irradiator is out of order.Not much progress due to low transformation efficienfy.
Week 5
Preliminary test (damage tolerance, Mitomycin C)
Not much progress due to low transformation efficienfy.Week 6
Damage tolerance assay (MitomycinC)
Week 7
Damage tolerance assay (MitomycinC and Hydrogen peroxide)
Week 8
Damage tolerance assay (Hydrogen peroxide)
Week 9
Logbook Entries
Click on any day in the calendar below to display detailed logbook entry.
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| week2 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | |||
| week3 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | |||
| week4 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | |||
| week5 | 26 | 27 | 28 | 29 | 30 | 31 | ||||
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| week7 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | ||||||
| week8 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | ||||||
| week9 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | ||||||
