The iGEM Team of University of Southern Denmark 2012 igem.sdu.2012@gmail.com

mRNA Isolation	PCR	Miniprep	Check Digest
3A-Assembly	Colony-PCR	Transformation	Gel-electrophoresis
Reverse Transcriptase	Mutagenisis	PCR-,gel clean-up	Content

Check Digest

Digest mix, using Fastdigest enzymes

Digest

1)Cast an agarose gel for purification of the cut product

2)Mix the digestion mixture in an eppendorf tube

3)Leave for 5 min. at 37°C (no shaking!)

4)Immidiately load the restriction mixture in the gel

5)Run the gel, cut out and purify bands of correct size.


Digestion Mix

4 µL H2O 
0,5 µL enzyme A
0,5 µL enzyme B
2 µL Fast Digest green buffer
3 µL dna product