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Team:SDU-Denmark/labwork/Protocols/Checkdigest
From 2012.igem.org
| mRNA Isolation | PCR | Miniprep | Check Digest |
| 3A-Assembly | Colony-PCR | Transformation | Gel-electrophoresis |
| Reverse Transcriptase | Mutagenisis | PCR-,gel clean-up | Content |
Check Digest
Digest mix, using Fastdigest enzymes
Digestion Mix4 µL H2O 0,5 µL enzyme A 0,5 µL enzyme B 2 µL Fast Digest green buffer 3 µL dna product
Digest 1)Cast an agarose gel for purification of the cut product 2)Mix the restriction mixture in an eppendorf tube 3)Leave for 5 min. at 37°C (no shaking!) 4)Immidiately load the restriction mixture in the gel 5)Run the gel, cut out and purify bands of correct size.
