Team:Freiburg/Project/Experiments
From 2012.igem.org
Gene activation
To show the functionality of our TAL protein as well as the impact of the VP 64 transcription factor fusion protein, we used a TAL-VP64 fusion construct targetting a minimal promotor coupled with EGFP.
![](http://imageshack.us/a/img854/1215/seaptest4.png)
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The experiment was done with four different transfections, either no plasmid, only the TAL vector, only the test plasmid or a cotransfection of both plasmids.
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![](http://imageshack.us/a/img854/1215/seaptest4.png)