Team:Goettingen/week18-3

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#3 Chemoreceptor Library - 18th Week

Inoculation of LB-media for qrtPCR of different promoter constructs

First Test for Isolaton of RNA, Synthesis of cDNA and qrtPCR approach

Experiment:
RNA was isolated using Nuceleus Spin RNA II (Machery and Nagel).
cDNA synthesis was performed using Reverse Transcription Kit (Qiagen).
First approach of qrtPCR with different delutions of cDNA in H20
For a detailed protocol click here.
Observations & Results
cDNA which was 1:10 diluted in H2O showed the best results for qrtPCR approach of our promoter constructs.

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