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Team:UC Chile/Cyano/Labook/april
From 2012.igem.org
April
Monthly Summary
PCR runs have been defective so we haven’t been able to obtain all the parts needed to assemble our constructs. Nonetheless, parts for C1.1 and C2.1 amplified and a Gibson assembly was attempted to join them. The assembly was incorrect according to a restriction enzyme digestion protocol.
Synechocytis are growing ok.
Synechocystis PCC6803
Attempted synechocytis DNA extraction according to protocol. There was no pellet and no DNA.
Synechocystis cultures were observed under acridine dye and turned out to be axenic. (upload photos). Cultures were reinoculated in fresh BG11.
Started a growth curve for Synechocystis (that was never finished).
PCR's
- 17 PCR’s were run. Some parts amplified correctly and some did not.
Comments: DNA from synechocystis as template is not good enough. Primers form secondary images of these structures)
