Team:UC Chile/Labook/march

March

Constructs and primers design

First constructs (link to constructs)

Primer sequences

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Strategy for integration construct: Part K292003 is not in the kit. It will be assembled from P1003+B0012+B0014

Getting parts ready

Plates prepared Broad host plasmid transformation Batch of new competent bacteria ready Primer alicuoting (primers asked for in late January).

Amplification of parts from 2011 kit: P1003, B0014 and others (specify) are needed for constructs. Standard Biobrick assembly.

                                                                                                              Transformation.

Plated, only one surviving colony.
Conclusion: psB1C3 was already CUT

                                                                                                                   PCR’s. By electrophoresis we have:

Unsuccessful attempts: psbAB,psbAB2, Kanr, RS1. Success: RFP, RS2, backbone. Comment: seems as primers for expression plasmid are problematic. Kanr new PCR attempts. Unsuccessful results. PCR for pPMQAK1, psbAB, RFP.

Work plan

Labour division: Bactomithril {Max, Emilia,Ulises, Claudia, Bryon}
Cyano {Simón, Carla, Tamara, Isaac, Sebastián}

Meetings with Dr. Gutiérrez every two weeks to check work done.