Team:Goettingen/week21-2

V09_17

• Experiment:
  In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .
  

• Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (Mg1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used:
  
  - 20E_flhDC
  - 18C_flhDC
  - 20I_flhdc
  - 20E_motA
  - 18C_motA
  - 20E motB
  - 18C_motB
  

Versuchsnummer

• Experiment:
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