Team:Goettingen/week10-2

V07_02

• Experiment:
  In order to clone the flhDC-promoter constructs intro pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restrictio products were subsequently separated on a gel in order to verify the digestions success and purification of the desired fragments.


• Observations & Results:
  The gel did not feature fragments of the expected size.Obviously, something was wrong with the digestion.

• Experiment:
  In order to gain more plasmid material of the flhDC-promoter constructs over night cultured were prepared for a subsequent plasmid isolation.
  20E - #2
  20G - #1
  2G - #1
  20I - #2
  18M - #2
  18O - #2
  18K - #1
  18C - #2
  

V07_03

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

• Experiment:
  The genes fliC (DH10B), fliC (Salmonella), motA, motB and yhjH were amplified by Pfu-Polymerase according to the following protocol. The success of the PCR was subsequently investigated preparing a 1% agarose gel.
  
• Observations & Results:
  The PCR failed for no bands were visible on the gel whereas the marker was clearly discernible.

V07_04

• Experiment:
  The genes fliC (DH10B), fliC (Salmonella), motA, motB and yhjH were amplified by Pfu-Polymerase according to the following protocol. Additionally, a negative control as well as a positive control were prepared this time. The success of the PCR was subsequently investigated preparing a 1% agarose gel.
  
• Observations & Results:
  The did not function again for no bands were visible on the gel; only the marker was clearly discernible. However, since also the positive control did not feature the slightest band, we assume that something might be wrong with our polymerase.

V06_15

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.