Team:Goettingen/week20-2

V09_10

• Experiment:
  Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)

• Experiment:
  Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.

• Experiment:
  MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).

• Experiment:
  Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed

V09_11

• Experiment:
  After the ligation of motA and motB into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.