Team:Goettingen/week9-2

V06_25

• Experiment:
  In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol.
  
• Observations & Results:
  The transformation failed. Only the positive control featured colony formation. We found out that accidentally ampicillin containing plates were used whereas the plasmid obtains a chloramphenicol resistance. Thus, the out come is not surprising

V06_26

• Experiment:
  Since we received a new E. coli (DH10B) strain, over night cultures were prepared in order to produce new competent cells.The following protocol was considered.
  
• Observations & Results:
  The performance of a test transformation proved the functionality of the new competent cells.

V06_19

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
• Observations & Results:
  The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.

• Experiment:
  Since the last time the fact that the araC-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol.
  
• Observations & Results:
  The transformation was again not successful. Only the positive control showed any growth.

V06_20

• Experiment:
  Over night cultures were prepared for group 1 and group 3:
  
  20E-flhDC
  18M-flhDC
  18C-flhDC
  
  20E-tar
  18M-tar
  19C-tar