Team:TU Darmstadt/Materials/PstI

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Contents

PstI

About

PstI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Providencia_stuartii Providencia stuartii 164]. It creates stick ends with 5' end overhang.

Working conditions

The recognition motive of EcoRI is: 

5' CTGCAG --> 5'---CTGCA     G---3'
3' GACGTC --> 3'---G     ACGTC---5'

Buffer: NEBuffer 3 + BSA

PstI requires at least 1bp overhang on its cutting site (with reduced cleavage efficiency 37%), more than 3bp (98%) are recommended.[1]

Supplier

  • NEB http://www.neb.com/nebecomm/products/productR3140.asp

References

[1] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp

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