Team:TU Darmstadt/Materials/PstI

From 2012.igem.org

Contents

PstI

About

PstI is a endonucleasic restriction enzyme isolated from Providencia stuartii 164. It creates stick ends with 3' end overhang.

Working conditions

The recognition motive of EcoRI is:

5' CTGCAG --> 5'---CTGCA     G---3'
3' GACGTC --> 3'---G     ACGTC---5'

Buffer: NEBuffer 3 + BSA

PstI requires at least 1bp overhang on its cutting site (with reduced cleavage efficiency 37%), more than 3bp (98%) are recommended.[1]

Supplier

References

[1] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp