Team:Goettingen/week20-2

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#2 Speed Improvement - 20th week

Mini Prep of Psb1c3 (including motA or MotB)

Test digestion of Psb1c3 (including motA and motB)

Experiment:
Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.

Digestion of motA, motB and the promoters 20E, 20I and 18C

Experiment:
MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).

Quickchange of FliC

Experiment:
Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed

Ligation of motA, motB into the Plasmid J61002 with the promoters 20E, 20I and 18C followed by transformation into DH10B

Experiment:
After the ligation of motA and motB into the plasmids with different promoters (20E, 20I and 18C) the ligation products were transformed into the [i]E. coli[/i] strain DH10B.

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