Team:TU Darmstadt/Materials

Materials

This page contains detailed instructions on the preparation of all employed materials.

Contents 1 Media 1.1 Defined Media 1.2 Standard Media 2 Antibiotics 3 Enzymes 3.1 DNA polymerases 3.2 DNA ligases 3.3 Restriction enzymes 3.4 Other 4 Proteases 5 Electrophoresis 5.1 Agarose gel electrophoresis 5.2 PAGE 6 Buffers 7 Acids and bases 8 Other common chemicals 9 Reporters 9.1 Dyes and stains 9.2 Fluorescent proteins 9.3 Enzymes 10 Detergents 11 Miscellaneous

Media

Defined Media

Standard Media

• DYT
• LB
• SOB
• SOC

Antibiotics

• Ampicillin
• Chloramphenicol
• Kanamycin
• Spectinomycin

Enzymes

DNA polymerases

• Phusion
• T4 DNA Polymerase

DNA ligases

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Restriction enzymes

Other

Proteases

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other common chemicals

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Detergents

Miscellaneous