Team:UIUC-Illinois/Notebook/Protocols/Storage

Select Protocol

• Bootcamp Protocols
• Subculturing Plates
• Making Electrocompetent E.Coli
• Making TAE Buffers
• Making Electrophoresis Gels
• Gel Purification
• Inoculation
• Miniprep
• PCR Protocols
• Digestions
• Ligations
• Transformation of E.Coli
• Storage of Cells

Storing cells in Glycerol Solution

What you need:

- 1.5 mL cryogenic vials
- 50% glycerol solution (can also use 20%)
- An overnight culture of the cells of interest

Procedure:

1. To a cryogenic vial, add 600 uL 50% glycerol solution and 400 uL liquid cell culture
2. Mix thoroughy and store in the -80˚C freezer until use

Unless noted, all procedures are based on those in the lab of Christopher Rao (http://www.scs.illinois.edu/rao/index.php).