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Team:KAIST Korea/Notebook Labnote/2012 10 temp
From 2012.igem.org
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Notebook : Labnote-October
Labnote
OctoberOctober 1st 2012
No Special Event!
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Back to the CalendarOctober 2nd 2012
No Special Event!
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October 3rd 2012
No Special Event!
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October 4th 2012
No Special Event!
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October 5th 2012
No Special Event!
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October 6th 2012
No Special Event!
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October 7th 2012
No Special Event!
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October 8th 2012
No Special Event!
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October 9th 2012
No Special Event!
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October 10th 2012
Flip FlopLuxI and Xis OE template preparation
Results
pAutoIntegrase cloning check and Xis template vector prep
Results
pAutoIntegrase 1-10 – HindIII cut
pGEM-Xis 1-2 – EcoRI cut
insert DNA preparation with OE PCR
Results
┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)
┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)
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October 11th 2012
Flip FlopAdditional templates
Results
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┗ Integrase and Excisionase template
October 12th 2012
No Special Event!
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October 13th 2012
No Special Event!
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October 14th 2012
Flip FloppAutoIntegrase Cloning
Insert DNA – OE PCR
Results
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Cloning into pSB4A5 is performed with gel extracted DNA fragment.
October 15th 2012
No Special Event!
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October 16th 2012
Flip FloppAutoSimple inserts nested PCR and aiiA template
Results
pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut
Results
single digestion with HindIII.
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October 17th 2012
Flip FloppAuto OE PCR and Xis, simple bFMO, and bFMO template preparation
Results
sizes ok
pAuto insert DNA preparation
Results
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intermediate fragment nested PCR
Full pAuto insert DNA construct OE PCR
October 18th 2012
Flip FloppAutoIntegrase enz cut check with NotI
Results
Lane 3, 6, 8 seem to be cloned.
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October 19th 2012
Flip FloppAutoIntegrase Alternative sequencing with fragments amplification
Results
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October 20th 2012
No Special Event!
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October 21st 2012
No Special Event!
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October 22nd 2012
No Special Event!
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October 23rd 2012
No Special Event!
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October 24th 2012
Flip FlopOE template preparation
Results
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gp47, aiiA, LuxI, bFMO for simple and pAuto
promoter and Inverted one
October 25th 2012
Flip FloppAuto intermediate fragment OE PCR
Results
OE PCR with new primer
Results
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pAutoSimple/inverted
pAuto/inverted
October 26th 2012
No Special Event!
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October 27th 2012
No Special Event!
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October 28th 2012
No Special Event!
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October 29th 2012
No Special Event!
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October 30th 2012
No Special Event!
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October 31st 2012
No Special Event!
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