Contents 1 Plasmid backbones 1.1 pSB1C3_IntK (BBa_K743006) 1.2 pSB1C3_IntS (BBa_K743010) 2 Biobricks and Constructs 2.1 BBa_K743000 2.2 BBa_K743001 2.3 BBa_K743002 2.4 BBa_K743009 2.5 BBa_K743016 2.6 BBa_K743014 2.7 BBa_K743015 2.8 BBa_K743018

Plasmid backbones

pSB1C3_IntK (BBa_K743006)

Neutral recombination plasmid backbone. Kanamycin resistance as a selectable marker for integration. EcoRI restriction site in RS2. Plasmid backbone designed for Gibson Assembly.

pSB1C3_IntS (BBa_K743010)

Suceptibility generating plasmid backbone. Spectinomycin resistance as a selectable marker for integration. mRFP1 under constitutive promoter in E.coli for negative selection of standard assembly.

Biobricks and Constructs

BBa_K743000

First recombination site of IntK plasmid backbone.

BBa_K743001

Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly.

BBa_K743002

Synechocystis PCC. 6803 Citocrome C promoter.

BBa_K743009

pSB1C3_IntK(BBa_K743006) with mRFP1 under PpsaAB promoter.

BBa_K743016

pSB1C3_IntK(BBa_K743006) with mRFP1 under PpsbA2 promoter.

BBa_K743014

pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminiscent under Transaldolase promoter.

BBa_K743015

pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under Transaldolase promoter.

BBa_K743018

pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under Transaldolase promoter.