"

From 2012.igem.org

• Home
• Team
  Biographies Facilitators The University(s) Official Profile
• Project
  Overview Data and Conclusions Future Directions References
• Parts
  Parts Submitted
• Lab Notebook
  Genetics Copper Etching Protocols
• Human Practice
  Outreach
• Safety
  Safety Security
• Sponsors
  Sponsor Us Acknowledgements
• iGEM

Data and Conclusions

The liquid media that Ferrooxidans thrive in is acidic so the copper team first started working on proving that the bacteria are doing the etching, not just the media. Our initial results were somewhat inconclusive, however the data showed that at certain time intervals the bacteria etched faster than the basal rate (see work from 7/7-7/17):

By furthur refining our experiments as described in the cooper notebook we were able to show that copper is in fact etched faster in the presence of A. Ferrooxidans than the basal rate of etching by the acidic liquid media (see the work done 7/18-7/22):

From this data we were able to conclude the following copper consumption rates:

The Copper team has further refined their experiments by adding more controls and nuances to the existing experiments in order to collect more refined data which is detailed in the notebook, however the major results are summarized here.

The genetics group intends to submit each of the following plasmids to the parts registry:

We plan to characterize parts BBa_K952006 and BBa_K952007 in ecoli.

For BBa_K95006 we can grow the cells in the absence of IPTG, then add the IPTG and shine blue light onto the culture to induce expression of the lysis cassette killing the cells. We will run 2 controls, the first we will add IPTG but keep the cells in the dark and the second we will shine blue light on the cells in the absence of IPTG. Neither of the controls should cause apoptosis while the culture with both promotors present should.

For BBa_K95007 we do not have the addition IPTG promotor present so we will grow the cells in the absence of light, then shine blue light on it to cause cell death. As controls we can attempt to grow the cells in the ambient light of the lab, under red light, and then shine blue light on these cultures if they grow. This is provide information on how sensative the system is to blue light. Ideally the cells would be able to grow in the ambient light of the lab with out inducing cell lysis however we do not expect this to be the case.