Team:ETH Zurich/Cph8

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Cph8

Figure: This figure shows the fusion of the Cph1 (green) with the PCB chromophore linked with the EnvZ Kinase  and OmpR (organe). Phosphorylated OmpR binds to the ompC promoter and activates transcription. [Levskaya2005]


Cph8 is a chimeric light receptor engineered by Levskaya et al. in 2005. It is a fusion of the photoreceptor Cph1 and the EnvZ histidine kinase.  The light sensing unit is connected to transcription via the EnvZ-OmpR signaling pathway, phosphorylated OmpR acting as a transcription factor. Both OmpR and EnvZ are endogenously expressed in E. Coli, so an envZ-deficient strain (e.g. JT2 or RU1012) has to be used. [Levskaya2005], [Tabor2011]

Cph1 is only active when it binds the chromophore phycocyanobiline (PCB). Since PCB is not naturally produced in E.coli we also need to express the PCB generators: ho1 and pcyA.

In this construct far red light activates the EnvZ kinase which results in the phosphorylation of OmpR and its activation as a transcription factor. Red light on the other hand deactivates the kinase and therefore the gene can not be expressed. 



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