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From 2012.igem.org

Abstract

Diarrheic pathogens including E.coli O157:H7 serotype, campylobacter, shigella, and salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths. Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization. Our method of bacterial detection fills a niche in biosensor technology. Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors. Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection. Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme. This functioning enzyme unit then cleaves x-gal and produces a colorimetric output signal. Our research demonstrates success in initial stages of chimeric protein assembly.

Website To Do List:

DO NOT DELETE THIS LIST! may be used for "Site Map". Due October 3, LESS THAN TWO WEEKS!!!

Project	Team	Results	Human Practices	Extras
make "Home" page pretty add info to "Problem" page make "Overview" page pretty add info to "Magainin" page add info to "Chimeric Reporter" page add info to "ssDNA" page	update bios on "Team" page	Get Lab Data add info to "Problem" page add info to "Data" page add info to "Main Results" page add info to "Judging Criteria" page add info to "Regional Jamboree" page	approve "University" page? approve to "Community" page? add info to "Regional Jamboree" page approve "International" page? add info to "Field Applications" page add info to "Modeling" page	approve "Safety" page? add info to "Site Map" page make banner/logo to put on top of page