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Team:XMU-China/futureplan
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Future Plan
Digital Display
Genetic parts we have constructed are more complicated than normal BioBricks, leading to uncertain performance which may not correspond to previous predictions. This problem highlights an issue that how to build robust and predictable synthetic gene networks. Unlike electronic circuits, most newly created gene circuits are non-functioning due to intrinsic parameter uncertainties, external disturbances and functional variations of intra- and extra-cellular environments[1]. In terms of molecular level, alteration in strength of the promoter and RBS is necessitated to obtain an optimal method for assemblage of genetic parts. Our goal for next stage is to seek a useful method for constructing a complex genetic network that can achieve its desired steady state behaviors. Once we achieve that, a real genetic seven-segment display will be much easier to build, so as other complex circuits.
Time Delay
Unsatisfying with our present stage of primary research of time delay, we plan to use the marker gene GFP and then evaluate the time spent for bio-circuits with different grade of RBSes to reach a certain fluorescent level. Besides, we also tend to characterize the control of pBAD and other promoter in a much more systematical way.
Cell Immobilization
We have almost finished building our display device, just a few more running tests are needed to improve and perfect it. We plan to immobilize cells that contain other genetic circuits we constructed and stuff them correspondingly into the seven glass tubes. The transportation system of oxygenated medium is also needed perfection. After some optimizing experiments, we can finally accomplish our digital display device.
Reference
[1]Chen, B.-S. and C.-H. Wu. A systematic design method for robust synthetic biology to satisfy design specifications[J]. BMC Systems Biology, 2009, 3(1): 66.
