Team:Goettingen/week18-2

V08_27

• Experiment:
  The Minipreps were conducted using the PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
  

• Experiment:
  The retransformation was performed as described in the standard protocol.
  
• Observations & Results:
  The retransformation was successful since numerous colonies could be obtained except for the empty negative control.

• Experiment:
  For we aimed to investigate the effect of our constructs on cells that are used to swim competent MG1655 cells were transformed with the following constructs:
  fliC (DH10B)
  fliC (Salmonella)
  motA
  motB
  yhjH
  
  18M-flhDC
  18O-flhDC
  18C-flhDC
• Observations & Results:
  The transformation was not successful. The next morning only one plate (the positive control) featured any visible colonies. However, after several additional hours of incubation also some very small colonies appeared at some other plates. Nevertheless, the efficiency was very low. We suggest that this is normal since MG1655 is no cloning strain and might have thus a much lower transformation efficiency. This idea was supported by our inquiries.

• Experiment:
  In oder to perform a Real-Time Quantitative Reverse Transcription PCR over night cultures of the tar-promoter constructs in BL21 were prepared.
  

V08_28

• Experiment:
  The flhDC-promoter constructs as well as the vector pSB1C3 still including tar were digested with EcoRI and PstI as described in the protocol. Afterwards, the fragments of the desired size were cut out and frozen in order to extract the DNA from the gel slices the following day.
• Observations & Results:
  The gel featured clearly visible bands of the expected length and thus the digestion was successful.

V08_29

• Experiment:
  TEXT

V08_30

• Experiment:
  TEXT