Team:Goettingen/week8-3

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#3 Chemoreceptor Library - 8th Week

Isolation of vector backbone pSB1C3 from BioBrick distribution kit

Experiment:
The vector pSB1C3 is used as the standard vector for BioBricks in this year's iGEM competition. We transformed E. coli DH10B with pSB1C3 according to protocol and streaked these out on Chloramphenicol (CM) plates.

V06_21_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3

Experiment:
All 8 promoter constructs + TAR_QC in J61002 and the BioBrick vector pSB1C3 were digested with XbaI and PstI according to protocol.

Observations and results:
The transformation of DH10B with pSB1C3 (see V06_18) worked well. pSB1C3 contained RFP which is now cut out.

V06_21_2 Preparation of glycerol stocks E. coli DH10B with pSB1C3

Experiment:
Glycerol stocks (30 %) were of 2 clones were prepared and stored at -80 °C.

Isolation of vector backbone pSB1C3 from BioBrick distribution kit

Experiment:
The vector pSB1C3 is used as the standard vector for BioBricks in this year's iGEM competition. We transformed E. coli DH10B with pSB1C3 according to protocol and streaked these out on Chloramphenicol (CM) plates.

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