Team:TU Darmstadt/Protocols/Affinity chromatography

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Affinity chromatography

Procedure

Column preparation

Before use the column needs to be activated:

  1. drain the ethanol
  2. wash the column with dist. water
  3. load the column with 12 mL NiCl2 solution
  4. wash the column with [ https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS buffer]

Now the column is ready to use.

Columns can be reused. In oder to store them:

  1. wash the coumn with EDTA buffer
  2. discard the flowthrough and wash two times with 20% ethanol
  3. close up the column with Parafilm and store at 4°C
Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography"