Team:Goettingen/week18-2

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#2 Speed Improvement - 18th week

Transformation of FlhDC-contructs into E.coli strain Mg

Experiment:
Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.

Test digestion of Psb1c3(including FlHDC under the control of different promoters) with

Experiment:
Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.

Biobrick Standardization of motA, motB and yhjH

Experiment:
PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).

Double Digest of motA, motB, yhjH and Psb1c3

Experiment:
MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).

Quickchange of FliC

Experiment:
Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.

Ligation and Transformation of motA, motB and yhjH

Experiment:
After the ligation of motA, motB and yhjH into the plasmid Psb1c3, the ligation products were transformed into the E. coli strain DH10B.

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