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Team:NTNU Trondheim/Stocks
From 2012.igem.org
This page lists stored cultures and other samples:
See also Constructs page.
Resuspended DNA (dried DNA from distribution kit resuspended in dH2O)
Isolated DNA (Miniprep, gel extraction and PCR products):
| Name/abbreviation | Date stored | Description | Concentration | Backbone |
|---|---|---|---|---|
| Lysis | 22.06 | BBa_K112808 | (RS) | pSB1A2 |
| LuxR+Term | 16.7 | |||
| LuxI+Term | 16.7 | |||
| C79 | 27.06 | <partinfo>BBa_C0079</partinfo> | 70,8 | pSB2K3 |
| C78 | 27.06 | <partinfo>BBa_C0078</partinfo> | 63,4 | pSB2K3 |
| RBS | 27.06 | <partinfo>BBa_B0034</partinfo> | 30,2 | pSB1A2 |
| GFP+Term | 11.07 | |||
| CFP+Term | 11.07 | |||
| LR | ? | <partinfo>BBa_C0062</partinfo> | (RS) | pSB1A2 |
| LI | ? | <partinfo>BBa_K092400</partinfo> | ||
| C78 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | |
| C79 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | |
| RBS | 24.06 | <partinfo>BBa_B0034</partinfo> | (RS) | pSB1A2 |
| YFP+Term | 11.07 | YFP+term (religated) | ? | |
| DLD + RBS | 9.07 | 24,1 | ||
| VHB + RBS | 9.07 | 48,3 | ||
| Jen1 + RBS | 9.07 | 35,3 | ||
| VGB + RBS | 9.07 | 40,0 | ||
| LR | ? | <partinfo>BBa_B0062</partinfo> | 20,3 | |
| VGB promoter | 27.06 | <partinfo>BBa_K561001</partinfo> | 22,9 | pSB1C3 |
| VGB DNA | 25.06 | <partinfo>BBa_K561001</partinfo> | pSB1C3 | |
| RFP +Term | 11.07 | |||
| Constitutive promoter | 22.06 | 27,7 | ||
| DLD | 4.07 | <partinfo>BBa_K284003</partinfo> | 63,8 | |
| K+RBS | 13.07 | <partinfo>BBa_K081005</partinfo> | 19,8 | |
| LuxR+HSL | 24.06 | |||
| pBAD+lysis | 9.7 | 23,6 | ||
| DNA lysis | 24.06 | |||
| D2 | 22.6 | BBa_K145150 | 23,1 | pSB1A2 |
| RBS | 6.07 | <partinfo>BBa_B0034</partinfo> | 44,5 | pSB1A2 |
| LuxI | 6.07 | 38,6 | ||
| VHB | 4.07 | <partinfo>BBa_K258005</partinfo> | 44,9 | pSB1A2 |
| Jen | 4.07 | <partinfo>BBa_K284002</partinfo> | 102,1 | |
| luxR+HSL | 25.06 | 13,3 | ||
| Lysis1 DNA | 24.06 | |||
| GFP+DTT | 13.07 | 41,1 | ||
| YFP+DTT | 13.07 | 55,5 | ||
| RFP+DTT | 13.07 | 40,9 | ||
| CFP+DTT | 13.07 | 47,7 | ||
| LuxI | ? | <partinfo>BBa_C0061</partinfo> | (RS) | |
| pBAD B1 | 1.07 | <partinfo>Bba_K206000</partinfo> | 24,0 | |
| D1 | 22.06 | <partinfo>BBa_K091146</partinfo> | 19,8 | pSB1A2 |
| K+RBS | ? | <partinfo>BBa_K081005</partinfo> | (RS) | pSB1A2 |
| pBAD B2 | 1.07 | <partinfo>Bba_K206000</partinfo> | 19 | pSB1A3 |
| Colicin | 4.07 | <partinfo>BBa_K150009</partinfo> | 58,6 | |
| VGB+RBS+YFP+DTT | 26.07 | |||
| VHB+RBS+YFP+DTT | 26.07 | |||
| EYFP+RBS+YFP+DTT | 26.07 | |||
| pTet GFP | 26.07 | <partinfo>BBa_I13522</partinfo> | pSB1A2 | |
| RBS | 26.06 | <partinfo>BBa_B0034</partinfo> | 16,8 | pSB1A2 |
| DTT Amp | 26.06 | <partinfo>BBa_B0015</partinfo> | 35,8 | pSB1AK3 |
| DTT Kan | 26.06 | <partinfo>BBa_B0015</partinfo> | 26,5 | pSB1AK3 |
| RFP K | 22.06 | |||
| GFP AK | 22.06 | |||
| CFP A | 22.06 | |||
| YFP AK | 22.06 |
Glycerol stocks:
| Name/abbreviation | Date stored | Strain | Plasmid | Position |
|---|---|---|---|---|
| pTet mRFP (BBa_I13521) | 27.07 | DH5a | pSB1A2 | |
| pTet GFP (BBa_I13522) | 27.07 | DH5a | pSB1A2 | |
| EYFP + LVA (BBa_E0430) | 27.07 | DH5a | pSB1A2 | |
| RFP (<partinfo>BBa_E1010</partinfo>) | 18.07 | DH5a | pSB2K3 | |
| VGB (<partinfo>BBa_K561001</partinfo>) | 18.07 | DH5a | pSB1C3 | |
| LuxR+HSL (<partinfo>BBa_R0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| Lysis (<partinfo>BBa_K112808</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| pBAD (<partinfo>Bba_K206000</partinfo>) | 16.07 | DH5a | pSB1A3 | |
| LuxR (<partinfo>BBa_C0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| GFP (<partinfo>BBa_K082003</partinfo>) | 16.07 | DH5a | pSB1AK3 | |
| pBAD+Lysis | 16.07 | DH5a | pSB1A3 | |
| DTT (<partinfo>BBa_B0015</partinfo> | 17.07 | DH5a | pSB1AK3 | |
| D2 (<partinfo>BBa_K145150</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| CFP (<partinfo>BBa_E0020</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| YFP (<partinfo>BBa_E0030</partinfo>) | 17.07 | DH5a | pSB1AK3 |
