Contents 1 Construction 1.1 Plasmids backbones 1.2 Constructs for Synechocystis 2 Characterization in Synechocystis 2.1 Transformation 2.2 Verification of constructs 2.3 Bioluminescence Assays 2.3.1 Bioluminometer 2.3.2 High-sensitive camera 3 Experimental Highlights

Construction

Plasmids backbones

We built two integrative plasmid backbones for Synechocystis PCC. 6803.

[File:UC_Chile-IntKplasmidResult.jpg| 480px | left] [File:UC_Chile-IntSplasmidResult.jpg| 480px | right]

Constructs for Synechocystis

Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)

[File:UC_Chile-IntKfullplasmidResult.jpg| 480px | left] [File:UC_Chile-IntSfullplasmidResult.jpg| 480px | right]

Characterization in Synechocystis

Transformation

We used our plasmid backbones to transform Synechocystis PCC. 6803.

-Image of Syne plates with colonies-

Verification of constructs

We proceeded to verificate the integration of the constructs in Synechocystis.

(New image with illustration of verification from outer RS1 and RS2 - October 1X something)

Bioluminescence Assays

Bioluminometer

-graph-

High-sensitive camera

-image-

Experimental Highlights