From 2012.igem.org
July 2012
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August 2012
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September 2012
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October 2012
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June 07
Transformation (LSE)
Transformed DNA:
lacZ (well 4:12G, I732019)
p + lacO (well 1:6G, R0011)
Cells: neb10beta (donated)
Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
Controls: puc19, no DNA (8 plates)
June 08
Transformation results
puc19: growth
negative control: no growth
lacZ, lacO: possible small colonies
liquid culture in amp media (100 ug / ml):
no growth of lacZ, lacO
growth of puc19
June 12
DH5a Competent Cell Prep
Streak plated cells on LB no amp plate, let grow overnight
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