Team:ETH Zurich/Decoder/Design

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Navigate through Overview, Design and Results

Contents

Circuit Design

The presence of blue or red light leads to the activation of LovTAP and to the deactivation of Cph8, respectively. As a result the expression of lacI and tetR being controlled by the tryptophan repressor promoter and the red light promoter is terminated.


Dual input promoters

In our case we want the decoder (construct 1 construct 2) to have NOT logics. Therefore we designed 3 different dual input promoters, all of them derived from the λ-phage promoters PR and PL:

1. a) [http://partsregistry.org/Part:BBa_K909012 Modified PR promoter with operator sites OR1,OR2 and LacO1] 

   b)  [http://partsregistry.org/Part:BBa_K909016  Modified PR promoter with operator sites OR1,OR2 and LacO1/O2 ]

2. [http://partsregistry.org/Part:BBa_K909013 Modified PR promoter with operator sites OR1,OR2 and TetO1]

3. [http://partsregistry.org/Part:BBa_K909011 Modified PL promoter with operator sites TetO1 and LacO1]

Figure: .


Output: p-ABA

We constructed one [http://partsregistry.org/Part:BBa_K909015 pab operon] consisting of the pabA and pabB/C gene from Lactococcus lactis to overexpress p-ABA. Therefore we used the biobrick parts [http://partsregistry.org/Part:BBa_K137055 BBa_K137055] and [http://partsregistry.org/Part:BBa_S04039 BBa_S04039] as a template to construct the wished composites.

Biobrick [http://partsregistry.org/Part:BBa_K909014 K909014] will be used to show that p-ABA is produced, while the [http://partsregistry.org/Part:BBa_K909015 operon] can be cloned downstream to any promoter.

In a second step the pab operon will be cloned downstream of the [http://partsregistry.org/Part:BBa_K909011 PL promoter with operator sites TetO1 and LacO1]. In case blue and red light is present, p-ABA will be produced as a blue and red light response.

Eth2012pabagoal.jpg




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