iGEM 2012

Quantitative model interactive simulations

Based on experimental data, we constructed what we refer to as a quantitative model. Please see Quantitative and stability model for details.

• Mutual repressor switch simulation
  
• The positive feedback loop switch simulation
  

Mutual repressor switch

INPUT VECTORS DOSAGE

Construct	Dosage
[A]_pCMV_TAL-B:KRAB_BFP	ng
[B]_pCMV_TAL-A:KRAB_mCitrine	ng
pCMV_[PIR]_TAL-B:KRAB	ng
pCMV_[ETR]_TAL-A:KRAB	ng
pCMV_PIP:KRAB	ng
pCMV_E:KRAB	ng

(Enter dosages for corresponding plasmids.)

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TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)

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INDUCERS

Pristinamycin
Input times [min]
Durations [min]

Erithromycin
Input times [min]
Durations [min]

(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION

---

Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)

Positive feedback loop switch

INPUT VECTORS DOSAGE

Construct	Dosage
[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP	ng
[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine	ng
pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16	ng
pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16	ng
pCMV_PIP:KRAB_E:KRAB	ng

(Enter dosages for corresponding plasmids.)

---

TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)

---

INDUCERS

Pristinamycin
Input times [min]
Durations [min]

Erithromycin
Input times [min]
Durations [min]

(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

---

SIMULATION
Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)