Team:HKUST-Hong Kong/Achievement
From 2012.igem.org
ACHIEVEMENT
From the day when our project was first proposed in mid-March to the day when we stop wet lab work in mid-September, we, our 2012 HKUST iGEM team have been working on our project for almost six months. In our six-month iGEM life, we spent three months in wet lab.
We are proud to say that within the three months, we have achieved the following:
- Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of Bacillus subtilis. (BBa_K733007)
- Successfully constructed two parts for BMP-2 synthesis and excretion using signaling peptides YbdN and YdjM respectively. (BBa_K733016, BBa_K733017)
- Successfully constructed a cell growth inhibitory device for regulating BMP-2 production (BBa_K733012)
- Successfully characterized the low efficiency promoter pTms which was used to drive the expression of antitoxin YdcD. (BBa_K733009, BBa_K733018)
- Successfully characterized a xylose inducible promoter which was used to control the expression of BMP-2 and toxin YdcE.
- Successfully characterized the cell growth inhibition device.