Team:HKUST-Hong Kong/Glossary

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Team:HKUST-Hong Kong - 2012.igem.org

GLOSSARY

Signal peptide: transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.

YbdN: Signal peptide of protein YbdN in Bacillus subtilis.

ybdN: Gene that encodes YbdN.

YdjM: Signal peptide from protein YdjM in Bacillus subtilis.

ydjM: Gene that encodes YdjM.

BMP-2: Bone morphogenetic protein 2.

LytC: B. subtilis inherent cell-wall associated protein mediating cell-wall turnover.

lytC: Gene that encodes LytC.

(EAAAK)n type helical linker: Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.

RPMrel: a nine amino acid, disulfide-constrained peptide, CPIEDRPMC (RPMrel), that selectively stained HT29 colon carcinoma cells.

FLAG:  8 amino acid peptide tag used in attachment to the protein-of-interest to facilitate multiple assays.

PxylA: The region containing the sigma A binding region for the xylose inducible promoter.

XylR: The transcriptional regulator for PxylA promoter.

pTms: A low efficient constitutive promoter in E. coli and B. subtilis.

EndoA: an endoribonuclease can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).

ydcE (ndoA): Gene that encodes EndoA which cleave the transcript of threonyl-tRNA synthetase (thrS).

YdcD: Antidote that counteracts with EndoA.

ydcD (endB): Gene that encodes YdcD.