Team:Goettingen/week10-3

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Team Goettingen

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#3 Chemoreceptor Library - 10th Week

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V07_02

Startpoint of Saturated mutagenesis experiment!

Experiment:
We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances. A saturated mutagenesis PCR was performed to mutate five individual amino acid residues in the ligand binding site of TAR. The primers that are utilized are the following:
TARR6973Fw gcgcaGGAAAGGTCTCACTGAGTnnNTCAGCGGTAnnNATGATGATGGATTCCTCCAATCAACAAAG
TARR6973Rv gcgcaGAGTAGGTCTCATCAGGTTAATGCGCGTTTGCAG
TARY149F150T154Fw gcgcaGGAAAGGTCTCAGGAGCTnnNnnNGCTCAGCCAnnNCAGGGAATGCAAAATGCAATGGGCGAAG
TARY149F150T154Rv gcgcaGAGTAGGTCTCACTCCAGTATTGCCATAATCTAGGTAATC

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