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Team:UC Chile2/General Protocols
From 2012.igem.org
Media
LB media
- 1% (w/v) tryptone
- 0.5% (w/v) yeast extract
- 86 mM NaCl
- 10g Tryptone
- 5g Yeast extract
- 5g NaCl
- 15g Agar -Optional- /L
- Adjust pH to 7.5 with NaOH 1M
Per liter:
SOB media
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
- 15g Agar -Optional-
- Adjust pH to 7.5 with NaOH 1M
Per liter:
