Team:TU Darmstadt/Materials/XbaI

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Contents

XbaI

About

XbaI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Xanthomonas_badrii Xanthomonas badrii]. It creates stick ends with 5' end overhang.

Working conditions

The recognition motive of XbaI is:[1] 

5' TCTAGA --> 5'---T     CTAGA---3'
3' AGATCT --> 3'---AGATC     T---5'

Buffer: NEBuffer 2 + BSA

SpeI requires at least 1bp overhang on its cutting site (99% cleavage efficiency).[2]

Supplier

  • NEB http://www.neb.com/nebecomm/products/productR0145.asp

References

[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_T-Z#X

[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp

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