Team:Goettingen/Project

From 2012.igem.org

(Difference between revisions)

Revision as of 20:37, 24 August 2012

Language:

English,

Deutsch

Our Project

Our project was born from the idea to create a real champion: the fastest E. coli in the world. As funny as this may sound at first, soon we were at the development of an ambitious plan to create our "Homing Coli" and apply its speed for selective purposes. The ultimate goal was a fast swimming E. coli strain which would be able to recognize specific molecules on a mutagenized receptor and head towards gradients of these substances on swimming agar plates. If this approach worked, it might be put to use for the recognition of various molecules such as pollutants, toxins or even cancer cell markers. As our planning moved on, we soon created three different focus groups which should work in parallel on the biggest and most crucial components of our project.

The first group focuses on the creation of effective swimming motility assays. All kinds of different media and swimming agar plates were to be tested, because fast E. coli can only show their potential under the right conditions. Furthermore, an efficient selection system should be created in order to separate the fast E. coli from the slower ones and to test potential attractants for our swimmers.

Creation of a fast strain represents the main task for the second group. The main question here is: which genes have the potential to make our E. coli faster and how do they need to be regulated to achieve this? Naturally, genes that code for parts of the bacterial motor, the flagellum, were selected for testing as well as FlhDC, a master regulator for motility and chemotaxis. The output is then measured as motility on the first group's swimming plates.

The last group focuses on the directed mutagenesis of the aspartate receptor TAR. Thereby, a library of numerous different and new TAR receptors can be created. Some of these might exhibit the ability to recognize a specific substance of interest. E. coli strains possessing such mutated receptors can then be screened for homing ability towards a selection of chemical compounds.

These three groups would focus mostly on their separate projects during the early phases of lab-work and also plan their schedules independently to minimize frictional losses. But as time progresses and the first results are obtained the work of our focus groups overlaps more and more in order to achieve our ultimate goal: the creation of Homing Coli.

Chemotaxis

Under process...

Poster