Team:UIUC-Illinois/Notebook/Protocols/TAE

From 2012.igem.org

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Line 76: Line 76:
<h2>Making 50X TAE buffer</h2><br/>
<h2>Making 50X TAE buffer</h2><br/>
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What you need: <br/><br/>
+
What you need: <br/>
- Tris base<br/>
- Tris base<br/>
- Glacial acetic acid<br/>
- Glacial acetic acid<br/>

Revision as of 19:44, 1 June 2012

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Protocols

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Buffer Protocols

Making 50X TAE buffer


What you need:
- Tris base
- Glacial acetic acid
- Na2EDTA•2H2O
- MilliQ water

Procedure:

1. In a 1000mL pyrex bottle add and mix:
- 242 Tris base
- 57.1mL glacial acetic acid
- 37.2g Na2EDTA•2H2O
- 1000mL MilliQ water

2. Mix thoroughly, no sterilization necessary

Making 1X TAE buffer


What you need:
- 50X TAE buffer
- MilliQ water

Procedure:

1. Add 20mL 50X TAE buffer to 980mL MilliQ water and mix to make 1L of 1X TAE buffer stock

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