Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis

From 2012.igem.org

(Difference between revisions)
Line 79: Line 79:
- High-melt agarose<br/>
- High-melt agarose<br/>
- TAE buffer 1X<br/><br/>
- TAE buffer 1X<br/><br/>
 +
- Autoclaved container <br/>
Procedure:<br/>
Procedure:<br/>
Line 89: Line 90:
- Low-melt agarose<br/>
- Low-melt agarose<br/>
- TAE buffer 1X<br/><br/>
- TAE buffer 1X<br/><br/>
 +
- Autoclaved container <br/>
Procedure:<br/>
Procedure:<br/>
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/>
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/>

Revision as of 19:39, 1 June 2012

Header

Protocols

Select Protocol

Making Gel Electrophoresis gel solutions



To check PCR results


What you need:
- High-melt agarose
- TAE buffer 1X

- Autoclaved container
Procedure:
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box)
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

To do a gel purification


What you need:
- Low-melt agarose
- TAE buffer 1X

- Autoclaved container
Procedure:
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

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